Research Focus
Memory circuits: Developing and using novel genetic tools to map and manipulate brain circuits in learning and memory processes.

Principle Investigator
• Ikerbasque Research Professor (2016-)
Achucarro Basque Center for Neuroscience, Bilbao, Spain “Tripartite synapse and extracellular matrix in learning and memory processes”
• Group Leader (2012-2016) NeuroCure Cluster of Excellence, Charité-Universitätsmedizin, Berlin, Germany “Identifying, validating, and connectivity mapping a ‘minimal’ memory circuit”
• Group Leader (2007-2011) Max Planck Institute for Medical Research, Heidelberg, Germany ”Cortical circuits in associative learning and memory”

Research Training
• Postdoctoral fellow (2001-2006) – Max Planck Institute for Medical Research, Heidelberg “In vivo two-photon imaging of neuronal activity with genetically-encoded Ca2+ sensors”
• Postdoctoral fellow (1998-2000) – University of Heidelberg “In vivo bioluminescence imaging of regulated gene expression in living animals”
• Postdoctoral fellow (1993-1997) – Massachusetts Institute of Technology, Cambridge, USA
“Inducible control of gene expression in the mammalian brain”

• Dartmouth College, New Hampshire, USA Doctor of Philosophy, 1993, Biochemistry “Forward genetic and cloning ‘regulatory genes’ controlling cholesterol metabolism”
• Boston University, Boston, USA Bachelors of Arts, 1986, Chemistry and Biology

1. Lissek T, Obenhaus HA, Ditzel DAW, Nagai T, Miyawaki A, Sprengel R, Hasan MT. General Anesthetic Conditions Induce Network Synchrony and Disrupt Sensory Processing in the Cortex. Front. Cell. Neurosci. 10:64, 2016.
2. Dogbevia GK, Roβmanith M, Sprengel R, Hasan MT. Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain. Mol Ther Nucleic Acids. 5:e309, 2016.
3. Dogbevia G, Marticorena-Alvarez R, Bausen M, Sprengel R, Hasan MT. Inducible and combinatorial gene manipulation in mouse brain. Front. Cell. Neurosci. 9:142, 2015.
4. Hasan MT, Hernández-González S, Dogbevia G, Treviño M, Bertocchi I, Gruart A, Delgado-García JM. Role of motor cortex NMDA receptor for learning-dependent synaptic plasticity in behaving mice. Nat Commun 4:2258, 2013.
5. Margolis DJ, Lütcke H, Schulz K, Haiss F, Weber B, Kügler S, Hasan MT, Helmchen F. Reorganization of cortical population activity imaged throughout long-term sensory deprivation. Nat Neurosci. 15:1539-46, 2012.
6. Lütcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Meyer S, Gobel W, Yang Y, Tang W, Kugler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, Hasan MT. Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice. Front Neural Circuits 4, 9, 2010.
7. Wallace, DJ, Meyer S, Astori S, Yang Y, Bausen M, Kügler S, Palmer AE, Tsien RY, Sprengel R, Kerr JN, Denk W, Hasan MT. Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor. Nat Methods 5, 797-804, 2008.
8. Zhu P, Aller MI, Baron U, Cambridge S, Bausen M, Herb J, Sawinski J, Cetin A, Osten P, Nelson ML, Kügler S, Seeburg PH, Sprengel R, Hasan MT. (2007). Silencing and un- silencing of tetracycline-controlled genes in neurons. PLoS ONE 2, e533, 2007.
9. Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W. Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. PLoS Biol 2, e163, 2004.10. Hasan MT, Schonig K, Berger S, Graewe W, Bujard H. Long-term, noninvasive imaging of regulated gene expression in living mice. Genesis 29, 116-122, 2001.